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anti fancj  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti fancj
    Anti Fancj, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fancj/FANCD2+Rabbit+mAb/pmc13006209-83-43-44
    Average 94 stars, based on 31 article reviews
    anti fancj - by Bioz Stars, 2026-09
    94/100 stars

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    Expressing:

    Article Title: Tumor Treating Fields (TTFields), and their concomitant application with FOLFOX, are effective for the treatment of gastric cancer cells
    Article Snippet: Cells were acquired using flow cytometry and γH2AX positive cells analyzed using the FlowJo software ( for gating strategy). .. For determination of protein expression, cell extracts were prepared and subjected to western blot analysis (40 μg protein) as previously described ( , ), using the primary antibodies: anti-p21 (Santa Cruz, SC-6246, 1:500), anti-p27 (Santa Cruz, SC-56338, 1:500), anti-FANCD2 (Cell Signaling, 16323, 1:1000), anti-FANCJ (Cell Signaling, 4578, 1:1000), anti-BRCA2 (Cell Signaling, 10741, 1:1000), anti-GAPDH (Santa Cruz, SC-32233, 1:2000). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (Abcam, ab97023 or ab6721, 1:2000) and gel imager (GeneGnome XRQ, AlphMetrix Bitech).

    Western Blot:

    Article Title: Tumor Treating Fields (TTFields), and their concomitant application with FOLFOX, are effective for the treatment of gastric cancer cells
    Article Snippet: Cells were acquired using flow cytometry and γH2AX positive cells analyzed using the FlowJo software ( for gating strategy). .. For determination of protein expression, cell extracts were prepared and subjected to western blot analysis (40 μg protein) as previously described ( , ), using the primary antibodies: anti-p21 (Santa Cruz, SC-6246, 1:500), anti-p27 (Santa Cruz, SC-56338, 1:500), anti-FANCD2 (Cell Signaling, 16323, 1:1000), anti-FANCJ (Cell Signaling, 4578, 1:1000), anti-BRCA2 (Cell Signaling, 10741, 1:1000), anti-GAPDH (Santa Cruz, SC-32233, 1:2000). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (Abcam, ab97023 or ab6721, 1:2000) and gel imager (GeneGnome XRQ, AlphMetrix Bitech).

    Article Title: Client recruitment mechanism of the cytosolic Fe-S cluster assembly targeting complex
    Article Snippet: Proteins as high-confidence hits of interest were visualized both by dot plot generated in ProHits-viz program and with Cytoscape (Shannon et al, ), either individually or grouped as protein complexes and biological processes according to their reported functions. .. Antibodies used for immunoblotting were anti-CIAO1 (1:1000, Cell Signaling Technology #81376), anti-MMS19 (1:500, Santa Cruz Biotechnology sc-390658), anti-CIAO2B (1:1000, Cell Signaling Technology #86302), anti-β-Tubulin (1:1000, Cell Signaling Technology #15115), anti-DYKDDDDK Tag (1:1000, Cell Signaling Technology #14793), anti-FANCJ (1:1000, Cell Signaling Technology #4578), anti-rabbit IgG, HRP-linked antibody (1:2000, Cell Signaling Technology #7074), and anti-mouse IgG, HRP-linked antibody (1:2000, Cell Signaling Technology #7076). .. All statistical analysis was performed on the basis of three technical or biological replicates unless specially indicated, and analyzed with GraphPad Prism software.

    Article Title: Client recruitment mechanism of the cytosolic Fe-S cluster assembly targeting complex.
    Article Snippet: Proteins as highconfidence hits of interest were visualized both by dot plot generated in ProHits-viz program and with Cytoscape (Shannon et al, 2003), either individually or grouped as protein complexes and biological processes according to their reported functions. .. Antibodies used for immunoblotting were anti-CIAO1 (1:1000, Cell Signaling Technology #81376), anti-MMS19 (1:500, Santa Cruz Biotechnology sc-390658), anti-CIAO2B (1:1000, Cell Signaling Technology #86302), anti-β-Tubulin (1:1000, Cell Signaling Technology #15115), anti-DYKDDDDK Tag (1:1000, Cell Signaling Technology #14793), anti-FANCJ (1:1000, Cell Signaling Technology #4578), anti-rabbit IgG, HRP-linked antibody (1:2000, Cell Signaling Technology #7074), and anti-mouse IgG, HRP-linked antibody (1:2000, Cell Signaling Technology #7076). .. All statistical analysis was performed on the basis of three technical or biological replicates unless specially indicated, and analyzed with GraphPad Prism software.



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    (A) Schematic showing the layout of conserved domains and motifs in TOPBP1. Numbered boxes represent BRCT domains, with phospho-peptide binding domains in green and domains lacking phospho-peptide binding activity in grey. Names of known TOPBP1 binding partners are shown below the domains they interact with. <t>CBR,</t> <t>CIP2A-binding</t> region; AAD, ATR-activation domain. (B) Sequence alignments of the two TOPBP1 BRCT7-binding motifs in DDIAS and a known interaction partner, <t>FANCJ.</t> (C) Sequence alignments of the TOPBP1 BRCT5-binding motif in DDIAS and a known interaction partner, BLM. (D) AlphaFold 3 predictions showing conserved phospho-peptide motifs in DDIAS in complex with TOPBP1 BRCT domains.
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    Image Search Results


    (A) Schematic showing the layout of conserved domains and motifs in TOPBP1. Numbered boxes represent BRCT domains, with phospho-peptide binding domains in green and domains lacking phospho-peptide binding activity in grey. Names of known TOPBP1 binding partners are shown below the domains they interact with. CBR, CIP2A-binding region; AAD, ATR-activation domain. (B) Sequence alignments of the two TOPBP1 BRCT7-binding motifs in DDIAS and a known interaction partner, FANCJ. (C) Sequence alignments of the TOPBP1 BRCT5-binding motif in DDIAS and a known interaction partner, BLM. (D) AlphaFold 3 predictions showing conserved phospho-peptide motifs in DDIAS in complex with TOPBP1 BRCT domains.

    Journal: bioRxiv

    Article Title: DDIAS is a single-stranded DNA-binding effector of the TOPBP1-CIP2A complex in mitosis

    doi: 10.1101/2025.09.09.675193

    Figure Lengend Snippet: (A) Schematic showing the layout of conserved domains and motifs in TOPBP1. Numbered boxes represent BRCT domains, with phospho-peptide binding domains in green and domains lacking phospho-peptide binding activity in grey. Names of known TOPBP1 binding partners are shown below the domains they interact with. CBR, CIP2A-binding region; AAD, ATR-activation domain. (B) Sequence alignments of the two TOPBP1 BRCT7-binding motifs in DDIAS and a known interaction partner, FANCJ. (C) Sequence alignments of the TOPBP1 BRCT5-binding motif in DDIAS and a known interaction partner, BLM. (D) AlphaFold 3 predictions showing conserved phospho-peptide motifs in DDIAS in complex with TOPBP1 BRCT domains.

    Article Snippet: The following antibodies were used at the indicated dilutions: BLM (A300-110A, Bethyl Laboratories, 1/2000), BRCA1 (sc-6954, Santa Cruz Biotechnology, 1/200), BRCA2 (OP95, Merck, 1/2000), CIP2A (14805, Cell Signaling Technology, 1/1000), FANCJ (4578, Cell Signaling Technology, 1/500), GFP (11814460001, Roche, 1/5000), H2AX (NB100-383, Novus Biologicals, 1/5000), HaloTag (G9211, Promega, 1/1000), PLK1 (05-844, Merck, 1/4000), RAD9 (sc-8324, Santa Cruz Biotechnology, 1/1000), RPA2 (ab10359, Abcam, 1/10,000), TOPBP1 (A300-111A, Bethyl Laboratories, 1/1000).

    Techniques: Binding Assay, Activity Assay, Activation Assay, Sequencing